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Main sol. 1493

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Internal Solution-ID: S3061

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 0.50 g 0.5 5.952
NaCl 0.50 g 0.5 8.556
KH2PO4 0.40 g 0.4 2.939
(NH4)2SO4 0.20 g 0.2 1.514
MgSO4 x 6 H2O 0.10 g 0.1 0.831
CaCl2 0.01 g 0.01 0.09
Li2B4O7 0.01 g 0.01 -
FeEDTA solution 1.00 ml - -
Trace element solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Do not adjust the pH.
2 For plates add 8g/l Gelrite or 15g Phytagel and 1g/L MgCl. A carbon source is not required because the organism degrades the polysaccharides in Gelrite or Phytagel
3 For liquid medium use 0.8-2.0g/l Gelrite as carbon source (the medium remains liquid). Alternatively mannose (1g/l) may be used instead of Gelrite in liquid medium. Do not add mannose to solid media, which will inhibit growth.
1493 VERRUCOMICROBIA MEDIUM