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Trace element solution

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Internal Solution-ID: S3063

This solution is used in 2 media and was first defined in medium 1493.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.200 g 0.2 -
Na2SeO4 x 10 H2O 0.440 g 0.44 -
CoCl2 x 6 H2O 0.180 g 0.18 1.386
MnSO4 x H2O 0.120 g 0.12 0.71
Na2MoO4 0.160 g 0.16 0.777
Na2WO4 0.100 g 0.1 0.34
ZnSO4 x 7 H2O 0.540 g 0.54 1.878
AlCl3 0.040 g 0.04 0.3
NiCl2 x 6 H2O 0.025 g 0.025 0.193
H3BO3 0.110 g 0.11 1.779
CuSO4 0.210 g 0.21 1.316
MnCl2 0.190 g 0.19 1.51
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
2 Final pH 7.0 (with KOH).
1493 VERRUCOMICROBIA MEDIUM
1685 HALORUSSUS VARIUS MEDIUM (MSC 15.2)