Resazurin solution
Internal Solution-ID: S30
This solution is used in 5 media and was first defined in medium 28.
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Equipment needed: Autoclave
Ice bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Resazurin
(0,1%) |
0.5 | ml | 0.005 | 0.022 | |
| Distilled water | 450.0 | ml | - | - | |
| 1 Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. | |||||
| 2 Cool to room temperature under an atmosphere of N2/CO2 in an ice bath. | |||||