Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Proteose peptone | 20.000 | g | 20 | - | |
| Glucose | 18.000 | g | 18 | 99.911 | |
| Yeast extract | 2.000 | g | 2 | - | |
| Sodium citrate dihydrate | 1.000 | g | 1 | 3.4 | |
| MgSO4 x 7 H2O | 0.980 | g | 0.98 | 3.976 | |
| Na2HPO4 x 7 H2O | 0.355 | g | 0.355 | 2.501 | |
| KH2PO4 | 0.340 | g | 0.34 | 2.498 | |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.020 | g | 0.02 | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Adjust pH to 6.5, autoclave at 110°C or filter-sterilize | |||||
Media with this solution
| 1500 | ACANTHAMOEBA MEDIUM |