Main sol. 1505a
Internal Solution-ID: S3114
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.3 | 2.205 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
| MgSO4 x 7 H2O | 0.12 | g | 0.12 | 0.487 | |
| CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.204 | |
| NaCl | 50.00 | g | 50 | 855.578 | |
|
Tryptone
(BD Bacto) |
5.00 | g | 5 | 70.343 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Na2CO3 | 5.00 | g | 5 | 47.175 | |
| NaHCO3 | 18.00 | g | 18 | 214.268 | |
| Seven vitamins solution | 1.00 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sodium carbonate, sodium hydrogencarbonate and vitamins), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate and hydrogencarbonate, adjust pH to 8.5 - 9.0, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add vitamins from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. | |||||
Media with this solution
| 1505a | PROTEINIVORAX HYDROGENIFORMANS MEDIUM |