Main sol. 1508
Internal Solution-ID: S3116
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA) |
21.00 | g | 20.958 | - | |
| K2HPO4 | 0.10 | g | 0.1 | 0.573 | |
| NH4Cl | 0.10 | g | 0.1 | 1.866 | |
| NaNO3 | 0.05 | g | 0.05 | 0.587 | |
| Yeast extract | 0.05 | g | 0.05 | - | |
| Ferric citrate monohydrate | 0.01 | g | 0.01 | 0.038 | |
| Trace element solution SL-4 | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.50 | g | 1.497 | 14.124 | |
| Na-pyruvate | 1.00 | g | 0.998 | 9.069 | |
| Na2S2O3 x 5 H2O | 1.00 | g | 0.998 | 4.021 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.039 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, pyruvate, thiosulfate, vitamins and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate, thiosulfate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of pyruvate, thiosulfate and vitamins should be sterilized by filtration. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary. | |||||
Media with this solution
| 1508 | CALDERIHABITANS MEDIUM |