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Main sol. 1516

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Internal Solution-ID: S3140

Compound Amount Unit Conc. [g/L] Conc. [mM]
Liquid medium 1000.0 ml - -
1 Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
Filtrate of Desulfurococcus kamchatkensis (DSM18924) 10.0 ml - -
Wolin's vitamin solution 10.0 ml - -
Na2S x H2O 0.3 g 0.3 3.844
2 Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
1516 THERMOFILUM MEDIUM