1
Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room
temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum
bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from
sterile stock solutions:
2
Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
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