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Main sol. 1525

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Internal Solution-ID: S3155

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
HEPES buffer
(SIGMA) 		3.57 g 3.531 14.818
NH4Cl 27.00 mg 0.027 0.499
CaSO4 x 2 H2O 60.00 mg 0.059 0.345
MgSO4 x 7 H2O 0.10 g 0.099 0.401
NaCl 8.00 mg 0.008 0.135
KNO3 0.10 g 0.099 0.978
NaNO3 0.69 g 0.682 8.03
Na2HPO4 0.11 g 0.109 0.766
Modified Wolin's mineral solution 10.00 ml - -
D-Glucose 2.00 g 1.978 10.981
Trypticase peptone
(BD BBL) 		1.00 g 0.989 -
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except glucose, peptone and vitamins) and adjust pH to 8.1. Dispense medium under air atmosphere into culture vessels suitable for anaerobic cultivation (e.g., 10 ml medium per Balch-type tube), seal and autoclave. Add glucose, peptone and vitamins from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 7.5, if necessary.
1525 KALLOTENUE MEDIUM