Back to all solutions

Main sol. 1526b

Internal Solution-ID: S3159

Equipment needed: Autoclave Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA)
60.0 g 59.88 -
Wolfe's mineral elixir 1.0 ml - -
Trypticase peptone
(BD BBL)
1.0 g 0.998 -
Yeast extract
(OXOID)
1.0 g 0.998 -
Glucose 1.0 g 0.998 5.54
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
Na2CO3 1.0 g 0.998 9.416
Wolin's vitamin solution (10x) 1.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.499 2.841
Na2S x 9 H2O 0.5 g 0.499 2.078
Distilled water 1000.0 ml - -
1 Dissolve ingredients except carbonate, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary.
1526b KIRITIMATIELLA (TYG) MEDIUM