Main sol. 1526b
Internal Solution-ID: S3159
Equipment needed: Autoclave
Filter
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA) |
60.0 | g | 59.88 | - | |
| Wolfe's mineral elixir | 1.0 | ml | - | - | |
|
Trypticase peptone
(BD BBL) |
1.0 | g | 0.998 | - | |
|
Yeast extract
(OXOID) |
1.0 | g | 0.998 | - | |
| Glucose | 1.0 | g | 0.998 | 5.54 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.0 | g | 0.998 | 9.416 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.499 | 2.841 | |
| Na2S x 9 H2O | 0.5 | g | 0.499 | 2.078 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary. | |||||
Media with this solution
| 1526b | KIRITIMATIELLA (TYG) MEDIUM |