Main sol. 1526d
Internal Solution-ID: S3162
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 60.0 | g | 59.347 | 1015.52 | |
MgCl2 x 6 H2O | 6.0 | g | 5.935 | 29.192 | |
KCl | 1.5 | g | 1.484 | 19.902 | |
Na2SO4 | 1.0 | g | 0.989 | 6.964 | |
NH4Cl | 1.0 | g | 0.989 | 18.492 | |
CaCl2 x 2 H2O | 0.4 | g | 0.396 | 2.691 | |
K2HPO4 | 0.4 | g | 0.396 | 2.272 | |
Modified Wolin's mineral solution | 10.0 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 4.9e-4 | 0.002 | |
Na2CO3 | 1.0 | g | 0.989 | 9.332 | |
Na-acetate | 1.6 | g | 1.583 | 19.292 | |
Na2-fumarate | 6.4 | g | 6.33 | 39.556 | |
Seven vitamins solution | 1.0 | ml | - | - | |
Na2S x 9 H2O | 0.5 | g | 0.495 | 2.059 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients except carbonate, acetate, fumarate, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, fumarate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (fumarate and vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary. |
1526d | LIMISALSIVIBRIO (L21 LS) MEDIUM |