Main sol. 1533
Internal Solution-ID: S3175
Equipment needed: Anaerobic workbench
Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| NaCl | 25.0 | g | 25 | 427.789 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Cysteine HCl | 0.5 | g | 0.5 | 4.127 | |
|
Yeast extract
(Oxoid) |
1.0 | g | 1 | - | |
| Trace element solution SL-7 | 1.0 | mL | - | - | |
|
Resazurin
(0.1%) |
1.0 | mL | 1.0e-3 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Add after autoclave | |||||
|
MgCl2 x 6 H2O
(150.0 g/l) |
20.0 | ml/l | 3 | 14.756 | |
|
NaHCO3
(10%) |
20.0 | ml/l | 2 | 23.808 | |
|
Na2S x 9 H2O
(2%) |
20.0 | ml/l | 0.4 | 1.665 | |
|
Glucose
(1M) |
20.0 | ml/l | 3.603 | 20 | |
| 2 pH 7.0 | |||||
| 3 Prepare the medium without NaHCO3, Na2S x 9 H2O, MgCl2 x 6 H2O and glucose. Boil the medium in a water bath and cool under a stream of N2/CO2 (80/20). Add the NaHCO3 to the cooled medium (the pH should be 7.0) and dispense the medium into Hungate tubes or serum bottles under a stream of N2/CO2 (80/20). Seal the tubes under N2/CO2 (80/20) and autoclave. To the cooled medium at the appropriate amounts of Na2S x 9 H2O, MgCl2 x 6 H2O and glucose from sterile, anaerobic stock solutions. | |||||
Media with this solution
| 1533 | MARINIFILUM MEDIUM (IRD MEDIUM) |