Main sol. 1539
Internal Solution-ID: S3191
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
K2HPO4 | 0.05 | g | 0.049 | 0.284 | |
KH2PO4 | 0.09 | g | 0.089 | 0.654 | |
NH4Cl | 0.25 | g | 0.247 | 4.623 | |
MgSO4 x 7 H2O | 0.25 | g | 0.247 | 1.003 | |
CaCl2 x 2 H2O | 0.15 | g | 0.148 | 1.009 | |
Yeast extract | 1.00 | g | 0.989 | - | |
Trace element solution | 10.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
Na2CO3 | 2.00 | g | 1.978 | 18.665 | |
D-Glucose | 2.00 | g | 1.978 | 10.981 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.297 | 1.689 | |
Na2S x 9 H2O | 0.30 | g | 0.297 | 1.235 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except carbonate, glucose, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.5, if necessary. |
1539 | THERMOTOGA PROFUNDA MEDIUM |