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Pfennig's heterotrophic salts solution

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Internal Solution-ID: S31

This solution is used in 5 media and was first defined in medium 28.

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ammonium chloride 0.35 g 13.462 251.665
Ammonium acetate 0.25 g 9.615 124.746
Pyruvic acid sodium salt 0.25 g 9.615 87.381
Dextrose 0.25 g 9.615 48.521
MgSO4 x 7 H2O 0.50 g 19.231 78.023
KCl 0.35 g 13.462 180.571
KH2PO4 0.35 g 13.462 98.92
Trace element solution SL-12 B 1.00 ml - -
Distilled water 25.00 ml - -
1 Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle.
28 PFENNIG'S MEDIUM I
39 PFENNIG'S MEDIUM I WITH YEAST EXTRACT
43 PFENNIG'S MEDIUM I WITH SALT
46 PFENNIG'S MEDIUM I WITH SALT
867 RHODOBACTER VELDKAMPII MEDIUM