Main sol. 1556
Internal Solution-ID: S3214
1 Cultivation of Liberibacter sp. | |||||
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
ACES | 10.00 | g | 10 | 54.885 | |
Na2 alpha-ketoglutarate | 2.00 | g | 2 | 13.881 | |
KOH | 3.75 | g | 3.75 | 66.838 | |
Distilled water | 550.00 | ml | - | - | |
Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
2 adjust pH to 6.9, autoclave for 15 minutes and allow to cool. | |||||
3 Add aseptically | |||||
Fetal bovine serum | 150.00 | ml | - | - | |
TMN-FH Medium
(Sigma-Aldrich, T3285) |
300.00 | ml | - | - | |
4 For preparation of agar plates add 15 g agar per liter of medium. |
1556 | BM7 MEDIUM |