Main sol. 1561
Internal Solution-ID: S3231
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract | 0.30 | g | 0.3 | - | |
NH4Cl | 0.50 | g | 0.5 | 9.348 | |
KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
MgCl2 x 6 H2O | 0.30 | g | 0.3 | 1.476 | |
KCl | 0.50 | g | 0.5 | 6.707 | |
Na2S2O3 | 0.10 | g | 0.1 | 0.233 | |
NaCl | 0.50 | g | 0.5 | 8.556 | |
CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
Sodium acetate | 0.50 | g | 0.5 | 6.095 | |
Sodium malate | 0.50 | g | 0.5 | 2.808 | |
HEPES | 3.00 | g | 3 | 12.589 | |
Trace element solution | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 7.5. | |||||
2 Autoclave at 121°C for 15 min. Before inoculation add 1.0 ml/ l 5% sulfide solution pH 7.5 and 1.0 ml/l vitamin solution. |
1561 | Medium for broad range phototrophic bacteria |