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Main sol. 1582

Internal Solution-ID: S3269

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 13.5 g 13.5 99.203
Yeast extract 10.0 g 10 -
Glucose 10.0 g 10 55.506
Starch
(soluble)
10.0 g 10 -
Cysteine hydrochloride 1.0 g 1 6.344
Deionized water 1000.0 ml - -
1 Adjust pH to 6.6 with NaOH. Boil medium and cool under N2, distribute under N2 and sterilize by autoclaving at 121°C for 20 minutes.
1582 MELISSOCOCCUS PLUTONIUS MEDIUM