Main sol. 1584
Internal Solution-ID: S3274
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 8.80 | g | 8.67 | 148.356 | |
| KCl | 0.34 | g | 0.335 | 4.493 | |
| NH4Cl | 0.25 | g | 0.246 | 4.605 | |
| MgCl2 x 6 H2O | 4.84 | g | 4.768 | 23.455 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.148 | 1.005 | |
| K2HPO4 | 0.15 | g | 0.148 | 0.848 | |
|
Fe(NH4)2(SO4)2
(0.1% w/v) |
3.00 | ml | 0.003 | - | |
|
NiCl2 x 6 H2O
(0.1% w/v) |
9.00 | ml | 0.009 | 0.068 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Na2CO3 | 1.50 | g | 1.478 | 13.943 | |
| Na2-fumarate | 8.00 | g | 7.882 | 49.25 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, fumarate and vitamins) and sparge medium with 80% N2 and 20% CO2 gas mixture to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding fumarate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of fumarate and vitamins are sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2, if necessary. | |||||
Media with this solution
| 1584 | DESULFUROMONAS CARBONIS MEDIUM |