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Main sol. 1585

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Internal Solution-ID: S3275

Equipment needed: Serum bottle Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 3.00 g 3 21.133
KH2PO4 1.80 g 1.8 13.227
MgSO4 x 7 H2O 0.10 g 0.1 0.406
(NH4)2SO4 0.10 g 0.1 0.757
CaCl2 x 2 H2O 0.04 g 0.04 0.272
FeCl3 x 6 H2O 0.03 g 0.03 0.111
MnSO4 x 5 H2O 0.03 g 0.03 0.124
Na2S2O3 x 5 H2O 10.00 g 10 40.292
Distilled water 1000.00 ml - -
1 Dissolve ingredients except thiosulfate and adjust pH of medium to 6.5 -7.0. Dispense medium under air atmosphere into serum vials suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml bottles) and close with butyl rubber septa. After autoclaving complete medium with thiosulfate added from a stock solution sterilized by filtration and add 3% (v/v) sterile CO2 to the gas phase.
1585 SULFURIFERULA MULTIVORANS MEDIUM