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Main sol. 1589

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Internal Solution-ID: S3284

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.950 g 0.95 6.981
K2HPO4 0.600 g 0.6 3.445
NH4Cl 0.270 g 0.27 5.048
Na-propionate 0.480 g 0.48 4.997
Glucose 0.480 g 0.48 2.664
Fructose 0.480 g 0.48 2.664
Sodium succinate dibasic 0.480 g 0.48 2.962
MgSO4 x 7 H2O 0.025 g 0.025 0.101
CaCl2 x 2 H2O 0.010 g 0.01 0.068
Vitamine solution 1.000 ml - -
Chelated iron solution 1.000 ml - -
Trace element solution 0.100 ml - -
Distilled water 1000.000 ml - -
1 The pH of the final solution is adjusted to 6.3.
2 Note: Please autoclave the chelated iron solution before you add to the medium, so that solves everything!!!!
1589 modified BAP+ (GFS) MEDIUM FOR FRANKIA