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Main sol. 1170

Internal Solution-ID: S333

Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.997 5.724
2 1.00 g 0.997 9.861
3 11.00 g 10.967 103.475
4 2.00 ml 1.575 49.163
5 18.00 g 17.946 307.074
6 0.16 g 0.16 2.982
7 0.24 g 0.239 0.971
8 24.00 mg 0.024 -
9 Trace element solution SL-6 1.00 ml - -
10 100.00 mg 0.1 0.063
11 1000.00 ml 995.221 55243.1
12 , might need strain adjustment 20.00 mM - -
Instructions:
  1. pH 10.0
  2. Prepare the medium without methanol, vitamin B12, MgSO4 x 7 H2O and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20 mM sodium acetate instead of methanol.
1170 METHYLONATRUM MEDIUM