1
Dilute 100 ml NMS salt solution to 1 litre with distilled water and then add 1 ml of
FeNaEDTA solution and 1 ml of the trace elements and autoclave. Also autoclave the
phosphate buffer stock solution.
2
After cooling of the autoclaved solutions, add 20ml phosphate buffer stock solution to
the mineral solution.
3
Dispense 9ml distilled water into the growth vessels. Use sealed vessels it is
appropriate to add 10% methane and 1% CO2 gas to the gas phase and autoclave.
4
When the growth vessels is cool, 1.0 ml of the mineral medium and 0.1 ml
0.03 M Cerium-(III)-chlorid are added.
5
Adjust pH to 3.5 with 1M HCL to obtain the final mineral medium before inoculate.
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