Main sol. 1599
Internal Solution-ID: S3335
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.1 | 0.492 | |
| KCl | 0.2 | g | 0.2 | 2.683 | |
| NaCl | 4.2 | g | 4.2 | 71.869 | |
| Na2SO4 | 3.0 | g | 3 | 21.121 | |
| Yeast extract | 0.2 | g | 0.2 | - | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| NaHCO3 | 3.3 | g | 3.3 | 39.282 | |
| NaCO3 | 6.5 | g | 6.5 | - | |
| Na-acetate | 3.0 | g | 3 | 36.57 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonates, acetate and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then, add and dissolve bicarbonate and carbonate, adjust pH to 9.5, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add acetate and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Adjust pH of complete medium to 9.5 - 9.7, if necessary. | |||||
Media with this solution
| 1599 | CONTUBERNALIS MEDIUM |