Main sol. 1605
Internal Solution-ID: S3345
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 2.609 | g | 2.609 | 34.997 | |
| NH4Cl | 0.267 | g | 0.267 | 4.992 | |
| MgSO4 x 7 H2O | 172.536 | g | 172.536 | 700.011 | |
| K2HPO4 | 0.030 | g | 0.03 | 0.172 | |
| Na2SO4 | 19.886 | g | 19.886 | 140.003 | |
| Na2CO3 | 0.023 | g | 0.023 | 0.217 | |
| TES | 2.292 | g | 2.292 | 9.998 | |
| Yeast extract | 2.500 | g | 2.5 | - | |
| Fe(NH4)citrate | 6.000 | mg | 0.006 | - | |
| NaHCO3 | 0.840 | g | 0.84 | 9.999 | |
| BG-FPC (100x) | 1.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| Wolfe´s Vitamins | 1.000 | ml | - | - | |
| 1 Adjust pH to 8.0 | |||||
| 2 Sterilize at 121°C for 15 minutes. After sterilization add 1 ml/l filter-sterilized Wolfe´s vitamin solution. | |||||
Media with this solution
| 1605 | HLH-700 MEDIUM |