Main sol. 1615
Internal Solution-ID: S3360
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 20 mM K-P-Buffer | 1000.0 | ml | - | - | |
| 1 After sterilization add from sterile stock solutions: | |||||
|
MgSO4 x 7 H2O
(1 M) |
1.0 | ml | 0.246 | 1 | |
|
Sodium acetate
(1 M) |
10.0 | ml | 0.82 | 10 | |
|
CaCl2 x 2 H2O
(5%) |
1.0 | ml | 0.05 | 0.34 | |
| 2 might result in colloid formation, especially when added into hot medium, but dissolves after vigorous shaking | |||||
|
Na-aspartate
(2 M) |
1.0 | ml | 0.354 | 2 | |
| Trace element solution | 1.0 | ml | - | - | |
|
L-Methionine
(50 mM, filter sterilized, neutralised) |
1.0 | ml | 0.007 | 0.05 | |
| Vitamine mixture | 0.5 | ml | - | - | |
| 3 pH 7.0-7.2 | |||||
Media with this solution
| 1615 | Basic mineral medium |