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HEPES buffer solution
Internal Solution-ID: S3383
This solution is used in 4 media and was first defined in medium 1630.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaOH
(pellets) |
24.0 | g | 24 | 600.045 | |
|
HEPES
(free acid) |
238.4 | g | 238.4 | 1000.42 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Fist dissolve NaOH in ca 600 ml water, then add HEPES, adjust pH to 7.5 and fill up to 1000 ml volume. | |||||