MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 1633

Download this solution as

CSV JSON PDF

Internal Solution-ID: S3391

Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone
(Bacto) 		0.250 g 0.323 -
Yeast extract
(Bacto) 		0.250 g 0.323 -
HEPES 2.380 g 3.075 12.904
Mineral salts solution 20.000 ml - -
Trace element solution 1.000 ml - -
NaHCO3 0.025 g 0.032 0.384
CaCO3 0.025 g 0.032 0.323
CaCl2 x 2 H2O 0.015 g 0.019 0.132
MgSO4 x 7 H2O 0.049 g 0.063 0.257
KNO3 0.010 g 0.013 0.128
KH2PO4 1.400 mg 0.002 0.013
NH4Cl 0.530 mg 6.8e-4 0.013
Agar
(Bacto) 		15.000 g 19.38 57.622
Double distilled water 730.000 ml - -
1 Sterilize 20 min./121°C. After cooling to 60°C add:
N-Acetylglucosamine
(10% stock solution, 10 % stock solution) 		8.000 ml 0.8 3.616
Glucose
(2.5%, sterile-filtered) 		10.000 ml 0.25 1.388
Vitamin solution (double conc.)
(medium 621) 		5.000 ml - -
2 Adjust pH to 7.0 with 40% KOH.
1633 V2 MEDIUM