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Neutralized sulfide solution 3% (w/v)

Internal Solution-ID: S34

This solution is used in 14 media and was first defined in medium 28.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 3 g 30 124.905
Distilled water 100 ml - -
1 The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.
28 PFENNIG'S MEDIUM I
28a THIORHODOCOCCUS MEDIUM
28b THIORHODOCOCCUS MEDIUM II
29 PFENNIG'S MEDIUM II
39 PFENNIG'S MEDIUM I WITH YEAST EXTRACT
43 PFENNIG'S MEDIUM I WITH SALT
46 PFENNIG'S MEDIUM I WITH SALT
87 CHLOROFLEXUS MEDIUM (modified)
421 CHROMATIUM SALEXIGENS MEDIUM
422 AMOEBOBACTER MEDIUM
867 RHODOBACTER VELDKAMPII MEDIUM
901 ACIDOLOBUS ACETICUS MEDIUM
1761 PFENNIG'S MEDIUM III
1778 ANB