Main sol. 1641
Internal Solution-ID: S3406
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 1.0 | g | 1 | - | |
|
Proteose peptone
(Difco no. 3) |
1.0 | g | 1 | - | |
| Casamino acids | 1.0 | g | 1 | - | |
| Glucose | 1.0 | g | 1 | 5.551 | |
|
Starch
(soluble) |
1.0 | g | 1 | - | |
| Na-pyruvate | 0.6 | g | 0.6 | 5.452 | |
| K2HPO4 | 0.6 | g | 0.6 | 3.445 | |
| MgSO4 x 7 H2O | 0.1 | g | 0.1 | 0.406 | |
| Sodium pyruvate | 0.6 | g | 0.6 | 5.453 | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
Media with this solution
| 1641 | DOUBLE CONCENTRATED 830 + 0.06% SODIUM PYRUVATE |