Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.25 | g | 0.25 | - | |
|
Peptone
(Difco) |
0.50 | g | 0.5 | - | |
| Glucose | 0.10 | g | 0.1 | 0.555 | |
| HEPES | 1.95 | g | 1.95 | 8.183 | |
| MgSO4 x 7 H2O | 0.37 | g | 0.37 | 1.501 | |
| CaSO4 x 2 H2O | 0.43 | g | 0.43 | 2.498 | |
| Agar | 14.00 | g | 14 | 41.626 | |
| Distilled water | 1000.00 | ml | - | - | |
| Vitamin solution | 5.00 | ml | - | - | |
| 1 Adjust pH to 7.0 prior to the addition of agar. | |||||
| 2 After autoclaving and cooling to about 55°C, add 5 ml of vitamin solution. | |||||
Media with this solution
| 1646 | GEMMATIMONAS MEDIUM |