Main sol. 1650
Internal Solution-ID: S3414
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2S2O3 x 5 H2O | 2.50 | g | 2.5 | 10.073 | |
| NaNO3 | 0.85 | g | 0.85 | 10.001 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| KH2PO4 | 0.10 | g | 0.1 | 0.735 | |
| NH4Cl | 0.10 | g | 0.1 | 1.87 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| Trace Element Solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH 7.0-7.2. | |||||
| 2 Dispense the medium into sterile bottles. Fill 6 ml medium in 15 ml tubes, fill the headspace with a mixture of N2/CO2 (80:20) and seal the bottles with butyl rubber stoppers . Autoclave at 121°C for 15 min. Add following solutions aseptically: 1 M Bicarbonate solution (see below) 30 ml, Vitamin solution (see below) 1 ml, 1 M acetate solution (see below) 1 ml. Adjust pH 7.0-7.2 | |||||
Media with this solution
| 1650 | 1650: Medium for Strain J5B |