Main sol. 1661
Internal Solution-ID: S3446
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.499 | 3.663 | |
| NaCl | 25.00 | g | 24.925 | 426.51 | |
| Yeast extract | 2.50 | g | 2.493 | - | |
| NaHCO3 | 1.85 | g | 1.844 | 21.956 | |
| Na2CO3 | 1.20 | g | 1.196 | 11.288 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Fe(III) citrate
(19% Fe) |
7.30 | g | 7.278 | 29.714 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.199 | 0.981 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, carbonate, trace elements, ferric citrate, magnesium chloride and vitamins) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and carbonate, then adjust pH to 9.5 and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add ferric citrate (10% w/v stock solution; dissolve by boiling and adjust pH to 7.5-8.0), trace elements, selenite-tungstate solution, magnesium chloride and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas. Sterilize vitamins by filtration. Adjust pH of the complete medium to 9.5, if necessary. | |||||
Media with this solution
| 1661 | ALKALIPHILUS NAMSARAEVII MEDIUM |