Main sol. 1662
Internal Solution-ID: S3447
Equipment needed: Filter
Gassing station
Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.294 | 2.161 | |
| K2HPO4 | 0.3 | g | 0.294 | 1.689 | |
| NH4Cl | 0.5 | g | 0.49 | 9.164 | |
| KCl | 0.1 | g | 0.098 | 1.315 | |
| NaCl | 5.0 | g | 4.902 | 83.88 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.49 | 2.411 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.098 | 0.667 | |
|
Yeast extract
(OXOID) |
5.0 | g | 4.902 | - | |
|
Trypticase peptone
(BD BBL) |
5.0 | g | 4.902 | - | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
| Na2CO3 | 3.0 | g | 2.941 | 27.75 | |
| Na-crotonate solution (1 M) | 10.0 | ml | - | - | |
| Na2S x 9 H2O | 0.3 | g | 0.294 | 1.225 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, crotonate and sulfide), adjust pH to 8.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add crotonate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. The crotonate solution should be sterilized by filtration. Adjust pH of the complete medium to 9.0, if necessary. | |||||
Media with this solution
| 1662 | ALKALIPHILUS HYDROTHERMALIS MEDIUM |