Main sol. 1663
Internal Solution-ID: S3448
Equipment needed: Gassing station
Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 3.62 | g | 3.62 | 61.944 | |
| K2HPO4 | 0.26 | g | 0.26 | 1.493 | |
| (NH4)Cl | 0.08 | g | 0.08 | 1.496 | |
| NaNO3 | 0.85 | g | 0.85 | 10.001 | |
| Na2SO4 | 0.05 | g | 0.05 | 0.352 | |
| Trace element solution | 1.00 | ml | - | - | |
| Wolin's vitamin solution | 10.00 | ml | - | - | |
| Na-DL-lactate | 1.12 | g | 1.12 | 9.995 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| Distilled water | 936.50 | ml | - | - | |
|
NaHCO3
(8%) |
52.50 | ml | 4.2 | 49.996 | |
| 1 Dissolve ingredients (except vitamins and trace elements) in distilled water; boil for 1- 2 minutes and cool down to room temperature under 100% N2 gas, dispense in culture vessels and autoclave. After sterilization add NaHCO3, vitamins, and trace elements from sterile anoxic stock solutions prepared under 100% N2. Adjust pH to 8.2 and add sodium sulfide (Na2S) from a sterile anoxic solution to achieve a final concentration of 100 µM prior inoculation. | |||||
Media with this solution
| 1663 | BSM medium |