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Main sol. 1664

Internal Solution-ID: S3450

Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)Cl 0.30 g 0.3 5.609
KH2PO4 9.00 mg 0.009 0.066
K2HPO4 22.00 mg 0.022 0.126
Na2HPO4 x 7 H2O 35.00 mg 0.035 0.247
MgSO4 x 7 H2O 23.00 mg 0.023 0.093
CaCl2 28.00 mg 0.028 0.252
FeCl3 x 6 H2O 0.25 mg 2.5e-4 9.2e-4
Na-lactate 200.00 mg 0.2 1.785
Peptone 500.00 mg 0.5 -
Na2S2SO3 1.00 g 1 -
Trace element solution 25.00 μl - -
Distilled water 1000.00 ml - -
Standard vitamin solution 25.00 μl - -
1 Dissolve ingredients (except vitamins) in distilled water.
2 After sterilization add the 25 µl vitamin solution.
1664 Sphaerotilus natans subspec. sulfidivorans medium