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Main sol. 1675

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Internal Solution-ID: S3469

Equipment needed: Autoclave Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.329 6.151
KCl 0.33 g 0.329 4.413
MgCl2 x 6 H2O 0.33 g 0.329 1.618
CaCl2 x 2 H2O 0.33 g 0.329 2.238
KH2PO4 0.33 g 0.329 2.418
Na2SO4 2.80 g 2.792 19.654
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.499 2.076
Distilled water 1000.00 ml - -
1 Dissolve all ingredients except vitamins and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 4.5 with HCl, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 4.5 - 4.8, if necessary.
1675 DESULFOTHERMOBACTER MEDIUM