Main sol. 1683
Internal Solution-ID: S3477
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
30% Salt Water (1L)
(30%) |
829.0 | ml | - | - | |
|
23% MGM (100mL)
(23%) |
5.0 | ml | - | - | |
| Sodium pyruvate | 5.5 | g | 5.5 | 49.982 | |
| Distilled water | 155.0 | ml | - | - | |
| 1 Adjust pH to 7.5 with 1 M HCl or 1 M NaOH | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
| 3 Before inoculation add from sterile stock solutions: | |||||
|
NH4Cl
(1M) |
5.0 | ml | 0.267 | 5 | |
|
K2HPO4
(0.5M) |
2.0 | ml | 0.174 | 1 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Vit 10 | 3.0 | ml | - | - | |
Media with this solution
| 1683 | CM1A Halorubrum Medium |