Main sol. 1691
Internal Solution-ID: S3500
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase peptone | 17.0 | g | 17 | - | |
Soy peptone | 3.0 | g | 3 | - | |
D-Glucose | 2.5 | g | 2.5 | 13.877 | |
Agar
(Bacto) |
20.0 | g | 20 | 59.465 | |
Artificial Sea Water Solution 2x concentrated (ASW)
(or Biomaris) |
1000.0 | ml | - | - | |
1 Adjust the pH to 7.3 with NaOH 4M | |||||
2 Add to 1000 ml of medium after autoclaving and cooling to 55°C: | |||||
Trace element solution SL-10 | 1.0 | ml | - | - | |
Vitamin solution | 1.0 | ml | - | - |
1691 | Artificial sea water - Tryptone soy broth Medium (ASW TSB Medium) |