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Main sol. 1691

Internal Solution-ID: S3500

Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D-Glucose 2.5 g 2.5 13.877
Agar
(Bacto)
20.0 g 20 59.465
Artificial Sea Water Solution 2x concentrated (ASW)
(or Biomaris)
1000.0 ml - -
1 Adjust the pH to 7.3 with NaOH 4M
2 Add to 1000 ml of medium after autoclaving and cooling to 55°C:
Trace element solution SL-10 1.0 ml - -
Vitamin solution 1.0 ml - -
1691 Artificial sea water - Tryptone soy broth Medium (ASW TSB Medium)