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Main sol. 1692

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Internal Solution-ID: S3504

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 0.60 g 0.6 3.33
Yeast extract 0.60 g 0.6 -
Tryptone 0.60 g 0.6 8.441
Starch
(soluble) 		0.60 g 0.6 -
Casamino acids 0.60 g 0.6 -
Sodium pyruvate 0.30 g 0.3 2.726
K2HPO4 0.30 g 0.3 1.722
MgSO4 x 7 H2O 0.05 g 0.05 0.203
Agar 20.00 g 20 59.465
Distilled water 1000.00 ml - -
1 Final pH 7.0; adjust with crystalline K2HPO4 or KH2PO4 before adding agar.
2 Autoclave for 15 min at 121°C.
1692 THERMORUDIS MEDIUM (CFH2)