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Main sol. 1697

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Internal Solution-ID: S3515

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 16.00 g 15.968 273.239
Na2SO4 2.50 g 2.495 17.566
CaCl2 x 2 H2O 1.00 g 0.998 6.788
KCl 0.45 g 0.449 6.024
NH4Cl 0.25 g 0.25 4.664
K2HPO4 0.14 g 0.14 0.802
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
HEPES buffer
(1 M, pH 8.0) 		20.00 ml 4.766 20
MgCl2 x 6 H2O 6.00 g 5.988 29.454
NaHCO3 1.00 g 0.998 11.88
Na2S2O3 x 5 H2O 2.50 g 2.495 10.053
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 980.00 ml - -
1 Dissolve ingredients (except HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins), dispense medium in test tubes or Erlenmeyrer flasks to 20% of volume, close with screw caps and autoclave. Add HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 8.0.
1697 THIOSOCIUS MEDIUM