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Main sol. 1717

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Internal Solution-ID: S3554

Equipment needed: Filter Gassing station Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.330 g 0.33 2.425
NH4Cl 0.330 g 0.33 6.169
KCl 0.330 g 0.33 4.427
NaCl 10.000 g 10 171.116
MgCl2 x 6 H2O 0.330 g 0.33 1.623
CaCl2 x 6 H2O 0.033 g 0.033 0.151
NaHCO3 2.000 g 2 23.808
Yeast extract 0.050 g 0.05 -
Tryptone 2.000 g 2 28.137
Fructose 2.000 g 2 11.101
Resazurin 1.000 mg 1.0e-3 0.004
Modified Wolin's mineral solution 10.000 ml - -
Wolin's vitamin solution 10.000 ml - -
Na2S x 9 H2O 0.500 g 0.5 2.082
Distilled water 1000.000 ml - -
1 Dissolve ingredients (except carbonate, and sulfide), adjust pH to 8.0, boil for 10 min. and sparge medium with 100% N2 gas for 30 min to make it anoxic. Distribute under N2 into Hungate tubes (10 ml) and autoclave. Add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Filter sterilize vitamin solution and aseptically add 100 µl per Hungate tube. Adjust pH of the complete medium to 8.0, if necessary.
1717 PERLABENTIMONAS MEDIUM