Main sol. 1731
Internal Solution-ID: S3577
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 18.0 | g | 18 | 308.008 | |
| MgCl2 x 6 H2O | 6.0 | g | 6 | 29.513 | |
| KCl | 1.5 | g | 1.5 | 20.121 | |
| Na2SO4 | 1.0 | g | 1 | 7.04 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| CaCl2 x 2 H2O | 0.4 | g | 0.4 | 2.721 | |
| K2HPO4 | 0.4 | g | 0.4 | 2.297 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.5 | g | 1.5 | 14.153 | |
| L-Fucose | 0.5 | g | 0.5 | 3.046 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, fucose, vitamins, cysteine, and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fucose, vitamins, cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Before use adjust the pH of the complete medium to 7.3 - 7.5, if necessary. | |||||
Media with this solution
| 1731 | PONTIELLA MEDIUM |