Main sol. J19
Internal Solution-ID: S3642
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone , tryptic digest | 10 | g | 9.99 | - | |
|
Beef extract
(BD-Difco) |
5 | g | 4.995 | - | |
|
Yeast extract
(BD-Difco) |
5 | g | 4.995 | - | |
| Glucose | 10 | g | 9.99 | 55.451 | |
| K2HPO4 | 3 | g | 2.997 | 17.207 | |
| Tween 80 | 1 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 6.8. | |||||
| 2 After sterilization, aseptically add solutions of sodium ascorbate and L-cysteine x HCl to final concentration of 1.0% and 0.05%, respectively. Heat medium not freshly prepared in a steamer for 10 min before addition of the reducing substances. | |||||
Media with this solution
| J19 | BIFIDOBACTERIUM MEDIUM |