MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 29

Download this solution as

CSV JSON PDF

Internal Solution-ID: S37

This solution is used in 2 media and was first defined in medium 29.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 460 ml - -
Solution B 40 ml - -
Solution C 50 ml - -
Solution D 450 ml - -
Solution E 26 ml - -
Solution F 10 ml - -
1 Mix solution D, C and E. Bubble with CO2 in an ice bath under sterile conditions.
2 Fill 50 ml in each bottle of solution A. Before using add 4 ml solution B and 0.1 ml solution F.
3 Adjust the pH with filter-sterilised 1M Na2CO3 to 6.8 -7.1.
4 Fill in sterile, N2 gassed screw-cap tubes under N2 gas.
5 During the first 24 h, the iron of the medium precipitates in the form of black flocks. No other sediment should arise in the otherwise clear medium. Feed periodically with neutralized 3% solution of sodium sulfide to replenish sulfide and with other supplement solutions (see Ref. 3365).
29 PFENNIG'S MEDIUM II
40 PFENNIG'S MEDIUM II WITH SALT