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Solution II

Internal Solution-ID: S3717

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glycerol 6 ml - -
Malachite green
(2%)
6 ml - -
Whole egg 200 ml - -
1 Homogenize Solution II by stirring and filter through gauze. Mix solutions I and II, and sterilize at 90C for 60 min.
2 Comment: Commercial 1% Ogawa medium (Eiken or Nissui) is also available.
J88 1% OGAWA MEDIUM