Main sol. J146
Internal Solution-ID: S3792
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Polypeptone
(BD-BBL) |
5.00 | g | 5 | - | |
Heart Infusion Broth
(BD-Difco) |
5.00 | g | 5 | - | |
Yeast extract | 5.00 | g | 5 | - | |
Pectin | 0.80 | g | 0.8 | 4.121 | |
Glucose | 0.80 | g | 0.8 | 4.441 | |
Starch | 0.80 | g | 0.8 | - | |
Sucrose | 0.80 | g | 0.8 | 2.337 | |
Maltose | 0.80 | g | 0.8 | 2.337 | |
Sodium pyruvate | 0.80 | g | 0.8 | 7.27 | |
Ribose | 0.80 | g | 0.8 | 5.329 | |
K2HPO4 | 2.00 | g | 2 | 11.483 | |
NaCl | 5.00 | g | 5 | 85.558 | |
MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
L-Cysteine HCl x H2O | 0.68 | g | 0.68 | - | |
Clarified rumen fluid | 500.00 | ml | - | - | |
Distilled water | 500.00 | ml | - | - | |
Agar | 1.60 | g | 1.6 | 4.757 | |
1 Adjust pH to 7.0. Prepare medium anaerobically and dispense into rubber-stoppered tubes under oxygen-free N2. Add inactivated sterile rabbit serum to give final concentration in each tube of 5%. Add filter-sterilized thiamine pyrophosphate to each tube to give a final concentration of 0.75 mg/100 ml. Subculture with a Pasteur pipette (20-30 drops). Gas phase for culture system should be oxygen-free N2 or N2-CO2 (90:10). |
J146 | MODIFIED OTI MEDIUM |