Main sol. J151
Internal Solution-ID: S3802
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Salt base solution | 1000.0 | ml | - | - | |
| Trace minerals | 10.0 | ml | - | - | |
| Citric acid | 5.0 | mg | 0.005 | 0.026 | |
|
Yeast extract
(BD-Difco) |
1.0 | g | 0.99 | - | |
|
Bacto peptone
(BD-Difco) |
5.0 | g | 4.951 | - | |
|
Sulfur
(powder) |
30.0 | g | 29.703 | 926.192 | |
| Na2S x 9 H2O | 0.5 | g | 0.495 | 2.061 | |
| Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
| 1 Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 6.5, if necessary. | |||||
Media with this solution
| J151 | PYROCOCCUS MEDIUM |