Main sol. J197
Internal Solution-ID: S3860
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.75 | g | 0.75 | 5.511 | |
| K2HPO4 | 0.75 | g | 0.75 | 4.306 | |
| MgCl2 x 6 H2O | 0.36 | g | 0.36 | 1.771 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
|
Yeast extract
(BD-Difco) |
0.10 | g | 0.1 | - | |
|
Polypeptone
(Nihon Pharm. Co.) |
0.10 | g | 0.1 | - | |
| Sodium acetate | 2.50 | g | 2.5 | 30.477 | |
| Trimethylamine-HCl | 8.00 | g | 8 | - | |
| Trace mineral solution | 9.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| NaHCO3 | 0.80 | g | 0.8 | 9.523 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 981.00 | ml | - | - | |
| 1 Mix ingredients except NaHCO3, Trace vitamins, Na2S x 9H2O and cysteine x HCl x H2O, and autoclave under a N2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as an 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O and cysteine x HCl x H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O and cysteine x HCl x H2O solutions, and adjust pH to 6.8. | |||||
Media with this solution
| J197 | JCM MEDIUM No. 197 |