Main sol. J198
Internal Solution-ID: S3863
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.75 | g | 0.736 | 5.408 | |
| K2HPO4 | 0.75 | g | 0.736 | 4.226 | |
| MnCl2 x 4 H2O | 0.36 | g | 0.353 | - | |
| NH4Cl | 1.00 | g | 0.981 | 18.347 | |
| NaHCO3 | 4.00 | g | 3.925 | 46.727 | |
| Trace mineral solution | 9.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| Na2S x 9 H2O | 0.50 | g | 0.491 | 2.043 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.491 | - | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients, except NaHCO3, Trace vitamins, Na2S x 9H2O and L-cysteine x HCl x H2O, and autoclave under a H2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O and L-cysteine x HCl x H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add the NaHCO3 solution and Trace vitamins to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add sterile the Na2S x 9H2O and L-cysteine x HCl solutions, and adjust pH to 7.4. Pressurize inoculated bottles to 200 kPa H2-CO2 (80:20). | |||||
Media with this solution
| J198 | JCM MEDIUM No. 198 |