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Main sol. 11b

Internal Solution-ID: S387

Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.00 g 10 -
2 10.00 g 10 -
3 5.00 g 5 -
4 20.00 g 20 111.015
5 1.00 g 1 0.764
6 1.00 mg 1.0e-3 0.004
7 2.00 g 2 11.483
8 5.00 g 5 60.951
9 2.00 g 2 8.223
10 0.20 g 0.2 0.811
11 0.05 g 0.05 0.296
12 1000.00 ml 998.207 55408.8
13 0.50 g 0.5 2.847
Instructions:
  1. Cysteine is added after the medium has been boiled and cooled under CO2. Adjust pH to 6.2 - 6.5, distribute under N2 and autoclave.
11b MRS MEDIUM pre-reduced