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Trace element solution

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Internal Solution-ID: S3909

This solution is used in 3 media and was first defined in medium J231.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.640 g - -
MgSO4 x 7 H2O 6.200 g - -
MnSO4 x n H2O 0.550 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoCl2 x 6 H2O 0.170 g - -
CaCl2 x 2 H2O 0.130 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.050 g - -
AlK(SO4)2 x 12 H2O 0.018 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.011 g - -
NiCl2 x 6 H2O 0.025 g - -
1 Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.
J231 METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM
J1064 GEOSPOROBACTER MEDIUM
J1074 VULCANIBACILLUS B5 MEDIUM